FIGURE 7.

Glabridin (GLA) prevents NF-κB and STAT6 signaling in the colonic macrophage from the doxorubicin (DOX)-treated mice. A single dose of DOX (20 mg/kg) was intraperitoneally injected into the C57BL/6 mice to induce acute cardiotoxicity. GLA (15 and 30 mg/kg) or vehicle was intragastrically administered once daily for 12 days, starting 7 days before DOX injection. (A) TLR4, p65, p-p65, p-IκBα, and IκBα in the colonic tissues were determined by Western blot (n = 10). (B) p-STAT6 and STAT6 in the colonic tissues were determined by Western blot (n = 10). The values are presented as the mean ± SEM. ^#P < 0.05, ^##P < 0.01 vs. control, ^∗P < 0.05, ^∗∗P < 0.01 vs. DOX.