Table 1.
Selected identification of differentially proteins of A. hydrophila LP-2 in iron starvation conditions by iTRAQ labeling analysis.
| Accession | Protein description | Peptide matched | %Coverage | iTRAQ ratio (Repeat1) | P-Value (Repeat1) | iTRAQ ratio (Repeat2) | P-Value (Repeat2) | Homologous to ATCC7966 |
|---|---|---|---|---|---|---|---|---|
| ORF02943 | Hypothetical protein | 12 | 25.94 | 21.28 | 5.24E-03 | 58.08 | 4.31E-04 | AHA_1448 |
| ORF01839 | Periplasmic iron-binding protein FecB | 19 | 40.13 | 19.59 | 1.32E-03 | 41.30 | 1.31E-02 | AHA_1964 |
| ORF01830 | FIG00919923: hypothetical protein | 93 | 56.61 | 10.19 | 5.96E-12 | 9.82 | 3.70E-03 | AHA_2481 |
| ORF01609 | Putative extracellular serine protease | 3866 | 97.6 | 2.99 | 2.73E-07 | 4.06 | 3.79E-08 | AHA_2687 |
| ORF02793 | Periplasmic molybdenum-binding protein ModA | 18 | 43.8 | 2.33 | 1.40E-02 | 3.66 | 6.64E-03 | modA |
| ORF04443 | Outer membrane vitamin B12 receptor BtuB | 21 | 40.74 | 2.58 | 2.69E-02 | 2.36 | 1.93E-02 | hgpB |
| ORF00614 | FIG003551: hypothetical protein | 16 | 53.96 | 2.36 | 3.68E-02 | 1.84 | 2.88E-02 | AHA_3660 |
| ORF03355 | FIG00545237: hypothetical protein | 40 | 69.86 | 1.66 | 5.06E-03 | 1.98 | 3.45E-04 | AHA_1098 |
| ORF03641 | Extracellular zinc protease | 232 | 64.69 | 1.71 | 3.04E-04 | 1.91 | 3.43E-06 | AHA_0851 |
| ORF04406 | Putative lipase | 99 | 46.77 | 0.54 | 7.20E-03 | 0.52 | 3.82E-02 | AHA_0104 |
| ORF03115 | Sialidase | 174 | 74.79 | 0.51 | 4.07E-04 | 0.52 | 2.73E-02 | nanH* |
| ORF03984 | Microbial collagenase, secreted | 185 | 68.09 | 0.36 | 1.10E-05 | 0.58 | 3.44E-03 | AHA_0517 |
| ORF02720 | Substrate binding periplasmic protein MalE | 63 | 66.16 | 0.43 | 4.95E-04 | 0.34 | 2.03E-02 | AHA_1667 |
| ORF03869 | Type II secretory pathway, pullulanase | 118 | 58.08 | 0.20 | 3.02E-03 | 0.49 | 7.19E-03 | AHA_0628 |
| ORF01696 | Alanine racemase | 11 | 33.09 | 0.13 | 8.19E-03 | 0.44 | 2.38E-02 | alr-3 |
| ORF02315 | 6-phosphofructokinase | 9 | 29.1 | 0.10 | 4.93E-03 | 0.45 | 2.16E-02 | pfkA |
| ORF04420 | Porphobilinogen synthase | 15 | 42.18 | 0.24 | 2.45E-02 | 0.24 | 3.19E-02 | hemB |
| ORF00636 | tolB protein precursor | 6 | 22.9 | 0.22 | 3.64E-02 | 0.18 | 3.05E-02 | tolB |
| ORF03513 | Chitinase | 204 | 60.07 | 0.18 | 1.23E-03 | 0.20 | 2.21E-05 | AHA_0977 |
| ORF04186 | SSU ribosomal protein S13p | 5 | 37.29 | 0.14 | 7.62E-03 | 0.20 | 1.18E-02 | rpsM |
| ORF00322 | FIG00362058: hypothetical protein | 21 | 61.02 | 0.14 | 1.35E-02 | 0.18 | 2.27E-02 | AHA_3948 |
| ORF02209 | FabA | 6 | 42.47 | 0.15 | 1.10E-02 | 0.15 | 1.90E-02 | fabA |
| ORF03511 | Chitinase | 187 | 72.97 | 0.13 | 4.82E-04 | 0.16 | 2.88E-04 | AHA_0979 |
| ORF03029 | hypothetical protein | 37 | 30.65 | 0.11 | 1.33E-02 | 0.14 | 2.99E-02 | AHA_1406 |
| ORF02740 | Glycerol kinase | 5 | 14.15 | 0.06 | 1.07E-02 | 0.09 | 1.85E-02 | glpK |
| ORF03889 | Chitin binding protein | 360 | 78.69 | 0.05 | 6.62E-05 | 0.09 | 1.04E-04 | AHA_0610 |
| ORF01532 | Cytochrome c4 | 29 | 46.23 | 0.03 | 3.55E-02 | 0.06 | 4.50E-02 | AHA_2763 |
| ORF02296 | Chitinase | 218 | 80.29 | 0.02 | 7.62E-06 | 0.05 | 3.47E-06 | AHA_2363 |
| ORF01386 | Cold shock protein CspE | 5 | 50 | 0.02 | 1.88E-03 | 0.03 | 8.51E-03 | AHA_2864 |
| ORF04175 | CopG protein | 2 | 12.59 | 0.01 | 4.48E-02 | 0.01 | 4.30E-02 | AHA_0347 |
| ORF02395 | T1SS secreted agglutinin RTX | 5 | 9.742 | 0.01 | 4.51E-02 | 0.01 | 4.39E-02 | AHA_2463 |
| ORF01538 | Peptidyl-prolyl cis-trans isomerase PpiB | 6 | 39.39 | 0.01 | 3.72E-02 | 0.01 | 3.70E-02 | ppiB |
| ORF01093 | Peptidyl-tRNA hydrolase | 2 | 32.83 | 0.01 | 4.84E-02 | 0.01 | 4.83E-02 | pth |
*
homologous to A. hydrophila strain ATCC 39315.