Fig. 4.

Hemodynamic change upon piezo stimulus of mice at 2 or 6 weeks postsoft cranial window implantation. (a) Optical recording of intrinsic signal (ORIS) set up with single-whisker stimulation (C2 whisker, 4 s) by piezo actuator. A 570-nm bandpass filter was used. (b) Spatial activation map of ORIS imaging for single-whisker stimulation. (c) Graph of $\mathrm{\Delta }R/R$ change ($n=10$ in each group). (d) Peak value of $\mathrm{\Delta }R/R$ change ($n=10$ in each group, $p<0.001$). (e) Spatial extent map of $\mathrm{\Delta }R/R$ change at the activated site following piezo stimulus. The spatial extent map described the areas that have $\mathrm{\Delta }R/R$ change values within 50% of the peak response value of $\mathrm{\Delta }R/R$ in whole frames. (f) Pixel counts in the spatial extent maps ($p\text{value}=0.03546$). (g) The spatial extent map of $\mathrm{\Delta }R/R$ change of C1 and C2 whisker piezo stimulation of an animal 2 and 6 weeks postsoft cranial window installation (Top 50 = within 50% of the peak response value of $\mathrm{\Delta }R/R$, top 40 = within 40% of the peak response value of $\mathrm{\Delta }R/R$, top 30 = within 30% of the peak response value of $\mathrm{\Delta }R/R$).