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. 2019 Feb 15;9(2):20180064. doi: 10.1098/rsfs.2018.0064

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© 2019 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — Fluorescence-in-situ-hybridization staining of fixed multispecies biofilms showing the localization of T. forsythia ATCC 43073 WT (a) and the NulO deficient mutant T. forsythia ATCC 43037 ΔpseC (b). Red: T. forsythia; cyan: P. gingivalis (a)/ T. denticola (b); green: non-hybridized cells (DNA staining YoPro-1 + Sytox). A representative area for one disc each is shown with a top view in the middle panel and side views with the biofilm–disc interface directed towards the top view. Scale bars, (a) 20 µm and (b) 15 µm. Both T. forsythia strains can be clearly detected in the form of microcolonies at the surface of the biofilm. Alteration of the surface glycosylation does not influence the bacteria's capability to grow in the multispecies consortium.