Figure 1.

(a) Schematic of the build-up of HA brushes (not to scale). (b) Assembly of an HA brush; a biotinylated OEG monolayer on gold was formed ex situ and all subsequent assembly steps were followed by QCM-D. Normalized frequency shifts (Δf_i/i) and dissipation shifts (ΔD_i) at overtones i = 3, 7, 11 are presented. The start and duration of incubation of each sample (20 µg ml⁻¹ SAv and 5 µg ml⁻¹ b-HA) are indicated by arrows on top; during remaining times, the surface was exposed to sample preparation buffer (10 mM HEPES, 150 mM NaCl, pH 7.4). (Online version in colour.)