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Microglia are the main source of iNOS in HSV-1-infected brains. Representative confocal images of brain stems obtained at day 5 p.i. with HSV-1. Neurons were identified as NeuN+-positive cells, while astrocytes as GFAP+ and microglia as CD11b+ and costained for iNOS and HSV-1. Nuclei were counterstained with DAPI.
