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. 2019 Feb 11;2019:9053295. doi: 10.1155/2019/9053295

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Copyright © 2019 Guoyong Yu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overexpression of c-Met reduced effects of paeoniflorin on c-Met/RhoA/Limk1 signaling. T98G and U251 were transfected vector plasmid or c-Met overexpression plasmid and then treatment was with or without 10μM paeoniflorin for 24h. The activity of GTP-RhoA (a-h) and the protein expression of ROCK1, Limk1, and p-Limk1 (i-l) were examined. Control: vector plasmid; PF: 10μM paeoniflorin +vector plasmid; ex-c-Met: c-Met overexpressed plasmid; PF+ex-c-Met: c-Met overexpressed plasmid +10μM paeoniflorin. All tests were performed in triplicate and presented as mean ± standard error. ∗P < 0.05, compared with control group. ^#P < 0.05, compared with PF group.