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. Author manuscript; available in PMC: 2019 Feb 25.
Published in final edited form as: Methods Mol Biol. 2018;1721:167–177. doi: 10.1007/978-1-4939-7546-4_15

Figure 1.

Figure 1.

Lithium acetate transformation of S. pombe using pFL20 as a positive control. (A) A map of pFL20 showing the pBR322 backbone, the stabilization fragment (stb), autonomously replication sequence (ars), restriction sites, and selectable markers URA3 and Amp [12]. Eco RI sites are in red, Hind III in green, and the Pvu I site is in blue. (B) Results of transformation with pFL20 showing colonies on PMG –ura plates. Two amounts of the pFL20 transformation were plated, 10% and 90%. The no DNA control plate indicates there was no contaminating source of growth in the experiment.