Table 1:

Transformation Solutions

Solution	Stock Concentration	Working concentration
(A). Tris-EDTA with Lithium Acetate
1. Tris-base ethylenediamine tetra acetic acid   (dissodium salt dihydrate)	100X (1M Tris-HCl, pH 8.0) (100 mM EDTA)	1X (10 mM Tris-HCl, pH 8.0) (1 mM EDTA)
2. Lithium Acetate	1M	100 mM
(B). Polyethylene Glycol with Lithium Acetate and Tris-EDTA
1. Poly-ethylene Glycol (PEG) 3,350	44%	40%
2. Lithium Acetate	1M	100 mM
3. Tris-EDTA	100X	1X