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. 2019 Feb 18;10:92. doi: 10.3389/fphys.2019.00092

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Copyright © 2019 Pazo, Pérez-González, Oliveros, Huarte, Chavez and Nieto.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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hCLE controls cap-dependent translation. (A) In vitro transcribed luciferase RNA was left uncapped or capped and both RNAs used for luciferase determination by in vitro translation assay. (B) HEK293T cells were transfected with 0.5 μg/2 × 10⁵ cells of in vitro transcribed luciferase RNA uncapped or capped and 1.5 h post-transfection luciferase activity was analyzed. The graphic shows the percentage of translated uncapped or capped luciferase RNA in control (taking as 100%) and hCLE silenced cells. Four independent experiments with three technical replicates were done. ^∗∗∗p-value < 0.0005.