Table 1.
Treatment Groups and Pregnancy Outcomes for Dams and Offspring.
| Treatment Group | Dam | Female Offspring | Male Offspring |
|---|---|---|---|
| Control | C1 | 2 | 2 |
| C2 | 3 | 2 | |
| C3 | 1 | 3 | |
| C4 | 3 | 2 | |
| C5 | 0 | 3 | |
| C6 | 1 | 2 | |
| C7 | 0 | 2 | |
| C8 | ED 14-15 tissues | ED 14-15 tissues | |
| C9 | ED 14-15 tissues | ED 14-15 tissues | |
| Total | 10 | 16 | |
| Metformin → Birth | MB1 | 0 | 2 |
| MB2 (5 dead PND1) | 0 | 0 | |
| MB3 | 2 | 2 | |
| MB4 | 3 | 3 | |
| MB5 | 3 | 1 | |
| MB6 (2 dead PND1) | 0 | 0 | |
| MB7 | ED 14-15 tissues | ED 14-15 tissues | |
| MB8 | ED 14-15 tissues | ED 14-15 tissues | |
| Total | 8 | 8 | |
| Metformin → Wean | MW1 (4 dead PND1) | 0 | 0 |
| MW2 (not pregnant) | 0 | 0 | |
| MW3 | 4 | 0 | |
| MW4 | 1 | 3 | |
| MW5 (1 dead PND1) | 0 | 0 | |
| MW6 | 2 | 3 | |
| MW7 | 3 | 2 | |
| MW8 | ED 14-15 tissues | ED 14-15 tissues | |
| Total | 10 | 8 | |
Numbers are pups surviving to wean, weaned offspring were ear tagged and same-sex group housed until behavior tests, C = control, MB = metformin treatment → birth, MW = metformin treatment → wean, M = male, F = female