FIG. 2.
Peptide P3 inhibits FGFR3 phosphorylation at Tyr 724. Human LECs were grown in culture, pretreated with either the ddH2O peptide vehicle (control), peptide P3 (P3), or scrambled peptide (Sc), followed by treatment with FGF2 or FGF2 vehicle. Protein was extracted, separated through electrophoresis, and blotted. (A) Blotting for phospho-FGFR3 (pFGFR3), FGFR3, and β-actin. (B) Relative protein expression was quantified. Cells coincubated with FGF2+Sc demonstrated greater levels of pFGFR3 expression compared with control treated cells. P3 and FGF2+P3 treated cells were associated with the lowest amounts of FGFR3 expression. Whole FGFR3 expression was negligibly affected. Therefore, P3 appears to effectively inhibit FGFR3 activation even in the presence of FGF2. *Indicates a p-value < 0.05.