FIG. 4.

P3-mediated FGFR3 inhibition does not slow nonpromoted LEC migration but does slow 9-cisRA-induced LEC migration. LEC migration was performed as previously described.¹⁴ Human LECs were grown in culture, seeded, pretreated with either scrambled peptide (Sc) or peptide P3 (P3), then incubated in either vehicle control or 9-cisRA until a confluent monolayer was established. A gap in the confluent monolayer was then created with a pipette tip, and cell migration was photographed at 0, 24, and 48 hours. Two replicate wells were used for each treatment group. (A) Representative images of the cell migration gap at 0, 24, and 48 hours. Quantified remaining migration gap area at 24 hours (B) and 48 hours (C). Total gap area was not significantly different between EtOH, EtOH+Sc, and EtOH+P3 treated cells at 24 and 48 hours after gap creation. LECs incubated with 9-cisRA and 9-cisRA+SC demonstrated significantly increased LEC migration at 24 and 48 hours. Coincubation of 9-cisRA+P3 was associated with significantly decreased LEC migration compared with 9-cisRA and 9-cisRA+Sc at 24 hours and compared with 9-cisRA, 9-cisRA+Sc, and vehicle control at 48 hours. *Indicates a p-value < 0.05.