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. 2019 Feb 11;25(3-4):234–247. doi: 10.1089/ten.tea.2018.0083

FIG. 7.

FIG. 7.

Effect of sequential addition of zone-specific growth factors to the chondrogenic medium/TGF-β1 on SZP (a), Sox-9 (b), Col II (c), AGC (d), Col X (e), and ALP (f) mRNA expression, collagen content (g), GAG content (h), and ALP activity (i) of hMSCs encapsulated in f-CarMa hydrogel. Groups included hMSCs in f-CarMa hydrogel incubated in BM supplemented with 3 ng/mL TGF-β1 for 2 weeks followed by BM with 30 ng/mL TGF-β1 for 4 weeks (Ctrl, light grey), hMSCs in CarMa hydrogel incubated in chondrogenic medium supplemented with 3 ng/mL TGF-β1 and 100 ng/mL BMP-7 for 6 weeks (BMP-7, black), BM with 3 ng/mL TGF-β1 and 100 ng/mL BMP-7 for 2 weeks followed by BM with 30 ng/mL TGF-β1 and 100 ng/mL IGF-1 for 4 weeks (BMP-7/IGF, dark grey), BM with 3 ng/mL TGF-β1 and 100 ng/mL BMP-7 for 2 weeks followed by BM with 30 ng/mL TGF-β1 and 100 ng/mL IGF-1 for 2 weeks and BM with 30 ng/mL TGF-β1 and 2.5 μg/mL IHH for 2 weeks (BMP-7/IGF/IHH, medium grey). An asterisk (*) indicates a statistically significant difference (p < 0.05) between the test group and all other groups for a given time point. BM, basal medium.