Table 1.

Matrix Source, Cell Density and Growth Factors Used for Chondrogenic Differentiation of hMSCs to the Superficial Zone Phenotype, Calcified Zone Phenotype, and for Sequential Differentiation of hMSCs to Superficial, Middle, and Calcified Zone Phenotypes

Sample name	Matrix source	Cell density (million cells/mL)	Growth factors
Superficial zone
a-25	Adult cartilage	25	TGF-β1 (3 ng/mL), BMP-7 (100 ng/mL)
f-25	Fetal cartilage	25	TGF-β1 (3 ng/mL), BMP-7 (100 ng/mL)
a-60	Adult cartilage	60	TGF-β1 (3 ng/mL), BMP-7 (100 ng/mL)
f-60	Fetal cartilage	60	TGF-β1 (3 ng/mL), BMP-7 (100 ng/mL)
a-100	Adult cartilage	100	TGF-β1 (3 ng/mL), BMP-7 (100 ng/mL)
f-100	Fetal cartilage	100	TGF-β1 (3 ng/mL), BMP-7 (100 ng/mL)
a-200	Adult cartilage	200	TGF-β1 (3 ng/mL), BMP-7 (100 ng/mL)
f-200	Fetal cartilage	200	TGF-β1 (3 ng/mL), BMP-7 (100 ng/mL)
Calcified zone
a-15	Adult cartilage	15	TGF-β1 (30 ng/mL)
f-15	Fetal cartilage	15	TGF-β1 (30 ng/mL)
a-100	Adult cartilage	100	TGF-β1 (30 ng/mL)
f-100	Fetal cartilage	100	TGF-β1 (30 ng/mL)
a-15-IHH	Adult cartilage	15	TGF-β1 (30 ng/mL), IHH (2.5 μg/mL)
f-15-IHH	Fetal cartilage	15	TGF-β1 (30 ng/mL), IHH (2.5 μg/mL)
a-100-IHH	Adult cartilage	100	TGF-β1 (30 ng/mL), IHH (2.5 μg/mL)
f-100-IHH	Fetal cartilage	100	TGF-β1 (30 ng/mL), IHH (2.5 μg/mL)
Sequential addition of growth factors
			Days 1–14	Days 15–28	Days 29–42
BMP-7	Fetal cartilage	100	TGF-β1 (3 ng/mL)	TGF-β1 (3 ng/mL)	TGF-β1 (3 ng/mL)
			BMP-7 (100 ng/mL)	BMP-7 (100 ng/mL)	BMP-7 (100 ng/mL)
BMP-7/IGF	Fetal cartilage	100	TGF-β1 (3 ng/mL)	TGF-β1 (30 ng/mL)	TGF-β1 (30 ng/mL)
			BMP-7 (100 ng/mL)	IGF-1 (100 ng/mL)	IGF-1 (100 ng/mL)
BMP-7/IGF/IHH	Fetal cartilage	100	TGF-β1 (3 ng/mL)	TGF-β1 (30 ng/mL)	TGF-β1 (30 ng/mL)
			BMP-7 (100 ng/mL)	IGF-1 (100 ng/mL)	IHH (2.5 μg/mL)