Semiquantitative measurement of the ALP enzyme activity of multipotent mouse C3H10 fibroblasts (a, b) and mouse C2C12 myoblasts (c, d) at the surface of 3D-printed PCL/β-TCP scaffolds exposed and not exposed to E-beam, at days 1, 3, and 7, in NC (DMEM) and OCs (DMEM supplemented with 100 ng/mL BMP-2). (a, c) show images of the scaffolds after ALP staining at day 7. (b, d) present the evolution of the ALP level for each group at days 1, 3, and 7 (n = 5). ALP, alkaline phosphatase; NC, normal conditions; OC, osteogenic condition. *p < 0.05.