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Effect of Rho kinase inhibition on YAP and E-Cadherin expression. Representative images of topographies 1 (A), 2 (B), and 3 (C) after culturing keratinocytes for 48 h under vacuum pressure in FAD medium containing ROCK inhibitor. Cells were fixed and labelled with antibodies to YAP (red), E-cadherin (green), and DAPI as a nuclear counterstain (blue). Individual confocal images and Z-stacks (right hand side) are shown. ROCK, Rho kinase. Scale bars are 100 microns.
