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Characterization of myotubes at day 14.
Notes: Representative images of (A) a myotube cocultured with embryonic rat spinal cord and (B) an aneurally cultured myotube. Myotubes were stained for ryanodine receptor (RyR) (green) and dihydropyridine receptor (DHPR) (red); scale bars =7.5 µm. (C) The percentage of myotubes with triads from cocultured and aneurally cultured myotubes. Results are expressed as mean ± SD, n=10, analyzed with unpaired t-test; ****P<0.0001.
