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. 2019 Feb 13;12:417–429. doi: 10.2147/IDR.S179639

Figure 7.

Figure 7

Fatty acid metabolism and resistance to CAZ.

Notes: (A) Percent survival of VA-RCAZ in the presence of the indicated triclosan plus CAZ. Overnight VA-RCAZ were incubated with CAZ (1.5 μg/mL) plus triclosan (2.5, 5 μg/mL) in M9 minimal medium plus NaAc (10 mM) at 30°C for 6 hours. (B) Percent survival of VA-S incubated in LB medium with exogenous palmitate or stearate, and then in M9 medium with CAZ (1.5 μg/mL). Overnight VA-S, cultured in LB with 0.2 mM of palmitate or stearate, were incubated with CAZ (1.25 μg/mL) in M9 minimal medium plus NaAc (10 mM) for 6 hours. (C) Quantitative real-time PCR for expression of genes in fatty acid metabolism. Overnight VA-S and VA-RCAZ were diluted to 1:100 using fresh 3% NaCl LB medium and grown at 30°C for 3 hours. (D) Outline for expression of genes in fatty acid metabolism pathway. Red: increase, green: decrease. Results (A–C) are displayed as mean ± SEM, and significant differences are identified as determined by Student’s t-test. *P<0.05, **P<0.01. At least three biologic repeats were carried out.

Abbreviations: CAZ, ceftazidime; LB, Luria–Bertani; VA-RCAZ, ceftazidime-resistant Vibrio alginolyticus; VA-S, ceftazidime-sensitive Vibrio alginolyticus.