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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: J Pathol. 2018 May 28;245(3):324–336. doi: 10.1002/path.5085

Figure 5.

Figure 5.

Regulation of SSP gene expression by menin. (A) qRT-PCR data showing down-regulation of SSP gene expression after menin (MEN1) knockdown with two different shRNA constructs. (B) Western blot showing that menin knockdown leads to down-regulation of PHGDH. (C) qRT-PCR data showing no consistent changes in SSP enzyme gene expression after MLL1 knockdown with two different shRNA constructs. n=3 independent experiments. (D) ChIP data showing reduced H3K4me3 enrichment at the PHGDH promoter after MI-503 treatment. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by two-tailed, unpaired t-test. All statistical comparisons are made relative to control (shNS).