Figure 1.

The antibacterial activity of traditional and calcium silicate-based root canal sealers against E. faecalis. The eluate from traditional and calcium silicate-based root canal sealers before (A) or after setting (B) was prepared using a PBS, and E. faecalis was cultivated with and without the prepared eluate of various sealers at various concentrations in a 96-well polystyrene plate. The growth of E. faecalis was measured using a microplate reader at 600 nm. The experiments were conducted three times in duplicate, and data are represented as the mean ± S.D. * Statistically significant differences compared with cultures not treated with the spent culture medium (p < 0.05).