Figure 6.

MALAT1 knockdown inhibited the growth of TSCC xenograft tumors through upregulating miR-140-5p and downregulating PAK1 in vivo.

Notes: (A) Tca8113 cells were infected with sh-NC or sh-MALAT1 lentiviruses. At 72 hours post-infection, MALAT1 level was determined by RT-qPCR assay. (B) Tumor volume was monitored using a caliper every week for a total of 5 weeks after injection. (C) Tumors were resected, photographed, and weighed at 5 weeks after injection. (D and E) The levels of MALAT1 and miR-140-5p were measured by RT-qPCR assay in TSCC xenograft tumors at the end of experiments. (F) PAK1 protein level was determined by Western blot assay in TSCC xenograft tumors at the end of experiments. ^*P<0.05.

Abbreviations: RT-qPCR, quantitative reverse transcriptase PCR; sh-MALAT1, MALAT1 shRNA; sh-NC, control shRNA; TSCC, tongue squamous cell carcinoma.