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. 2019 Feb 18;12:1309–1318. doi: 10.2147/OTT.S184560

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© 2019 Zhao et al. This work is published and licensed by Dove Medical Press Limited

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Knocking down NUCB2 in papillary thyroid cancer cells inhibited tumor cell proliferation by regulating Ki67 and PCNA.

Notes: (A) Typical images of colony-forming assay and its quantification demonstrated that colony rate of shRNA group was significantly lower than control group (n=3, P<0.05, respectively). (B) OD value of MTT assay suggested that cell proliferation rate of shRNA group was lower than control group (n=3, P<0.05, respectively). (C and D) Western blot and its quantification showed that the expression of Ki67 and PCNA was decreased in shRNA group when compared to control group (n=3, P<0.05, respectively). *P<0.05.