Figure 1.

IR increases PNUTS expression and induces the CNE-2 cell migration and invasion.

Notes: (A) After IR treatment, PNUTS expression in the HONE-1, CNE-2, and CNE-1 cell lines was detected by Western blotting. (B, C) The migration and invasion abilities of CNE-2 cells with or without IR treatment were measured by wound-healing assays (photographed at 100× magnification after 24 and 48 hours) and Transwell assays (photographed at 100× magnification after 24 hours). Mean ± SD. n=3. **P<0.01. GAPDH served as an internal control.

Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IR, ionizing radiation; PNUTS, phosphatase 1 nuclear-targeting subunit.