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. 2019 Feb 15;12:1205–1214. doi: 10.2147/OTT.S188571

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© 2019 Yu et al. This work is published and licensed by Dove Medical Press Limited

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Overexpression of PNUTS induces EMT and significantly increases the mobility and invasiveness of CNE-2 cells.

Notes: (A) CNE-2 cells were infected with ad-GFP or ad-PNUTS at an efficiency measured by Western blotting. Western blotting was used to detect the expression of vimentin, N-cadherin, and E-cadherin. The internal control was GAPDH. (B, C) After overexpressing PNUTS, wound-healing assays (100× magnification) and Transwell assays (200× magnification) were used to detect the mobility and invasiveness of CNE-2 cells. Mean ± SD. n=3. *P<0.05, **P<0.01 vs control group.

Abbreviations: EMT, epithelial–mesenchymal transition; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GFP, green fluorescent protein; PNUTS, phosphatase 1 nuclear-targeting subunit.