Table 1.
Main features and genetic abnormalities of the neuroblastoma cell lines used in this study
| Cell line | Cell type | Age/sex | Genetic abnormalities |
|---|---|---|---|
| SK-N-AS | Neuroblastoma Bone marrow metastasis Poorly differentiated |
6/F | FLT3 Het G739R;20 IGF-I expression;21 No/low MDR1 expression;21 MYCT1 overexpression;20 NRAS Het Q61K;20 Rb Het L477P20 |
| SK-N- BE(2) | Neuroblastoma Bone marrow metastasis Previously treated |
2/M | Analysis of clone M17 in COSMIC:20 AKT3 overexpression;20 MYCN amplification;20 NF1 deletion-frameshift N664fs*1;20 p53 Hom C42F, C135F20 |
| IMR5 | Neuroblastoma Primary | 1/M | AKT3 overexpression;20 ALK copy number gain;20 mTOR Hom F1888V;20 MYCN amplification20 |
| LAN1 | Stage IV Neuroblastoma Bone marrow metastasis |
2/M | Not available in COSMIC.20 ALK F1174L;22 MYCN amplification;23 p53 nonsense mutation at cysteine 182, absence of protein expression24 |
| SK-N-SH | Neuroblastoma Bone marrow metastasis |
4/F | ALK Het F1174L, overexpressed;20 MYCT1 overexpression20 |
| SHEP | Neuroblastoma Lacks most neuronal characteristics Derived from SK-N-SH Previously treated |
4/F | Not available in COSMIC:20 Derived from SK-N-SH. 47,XX,der(1)inv dup(1)(q11q24) inv(1) (q25q44)dup91 (q25q44),+7,add(9)(q34),der(22)t(17;22)(q21;q13)21 |
| SK-N-MC | Neuroepithelioma Metastatic |
14/F | Normal chromosomes N3 and N10 are absent, and many (N1, N2, N4, N15, N16, N17, N21, and N22) are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. Numerous marker chromosomes are present including: 1p+, der(3)t(2;3)(q24;q27), del(4)(p12), 11q+, del(2)(q23), ampl.(17)(p12), ampl.(16) (q13), del(15)(q13q22), 21p+, iso(3q), del(22)(q11q13)21 Analysis of clone MC-IXC in COSMIC:20MYC copy number gain;20 PTEN copy number loss;20 Rb Hom R698M/S (2 different substitutions at same codon);20 p53 Het c.1_169del39520 |
Abbreviations: add, addition; ampl, amplification; COSMIC, catalog of somatic mutations in cancer; del, deletion; der, derivative; dup, duplication; F, female; Het, heterozygous; Hom, homozygous; ins, insertion; inv, inversion; iso, isoform; M, male.