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. 2019 Feb 18;11:1601–1613. doi: 10.2147/CMAR.S191102

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© 2019 He et al. This work is published and licensed by Dove Medical Press Limited

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PCDH17 ectopic expression promotes cell cycle arrest and induces apoptosis in NPC cells.

Notes: (A) Flow cytometry was used to determine the cell cycle distribution of CNE1-vector cells and CNE1-PCDH17 cells. The percentage of S phase cells is shown.

*P<0.05. (B) The Annexin-V kit was used to detect the apoptosis of CNE1-PCDH17 cells and CNE1-vector cells. The percentage of early apoptotic cell numbers is shown.

*P<0.05. (C) Western blot was used to examine the expression of several key apoptosis and autophagic regulators.

Abbreviations: NPC, nasopharyngeal carcinoma; PE, phycoerythrin; PI, propidium iodide; 7-AAD, 7-amino-actinomycin D.