Table 1.

PCR primers used in this study

Genes	Forward		Reverse	Product size (bp)
RT-PCR
PCDH17	5′-TGGAGGAGAGGAACGCCATG-3′		5′-AACAAACTGCTGCCTGCTGC-3′	299
GAPDH	5′-GATGACCTTGCCCACAGCCT-3′		5′-ATCTCTGCCCCCTCTGCTGA-3′	304
For detecting methylated PCDH17 promoter by MSP
PCDH17		5′-GATTATCGGGTGTCGTAGTTC-3′	5′-CCCTAACGCAACGTACGCG-3′	87
For detecting unmethylated PCDH17 promoter by MSP
PCDH17		5′-AGATTATTGGGTGTTGTAGTTT-3′	5′-AACCCTAACACAACATACACA-3′	90
For BGS analysis of PCDH17 methylation
PCDH17		5′-TGAGTAGAATAAGGAGAGATTAT-3′	5′-ACAACTAACACTTAACATTATAAC-3′	490

Abbreviations: RT-PCR, reverse transcription PCR; MSP, methylation-specific PCR; BGS, bisulfite genome sequencing.