Figure 1.

Forskolin promoted the protein expression of cleaved caspase-3/9 and repressed cell growth in NHL cells.

Notes: (A, B) Toledo, NK-92, RL, and Farage cells were treated with 30 μM of forskolin for 48 hours, then the cells were harvested for Western blotting to assess the expression of total or cleaved caspase-3/9. (C) MTT assay was conducted to test the cell growth after Toledo cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 1, 2, 3, 4, 5, or 6 days. (D) Toledo cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 48 hours, then MTT assay was used to evaluate the IC₅₀ of forskolin. (E) Toledo cells were incubated with 0, 20, 40, 80, or 160 μM of forskolin for 48 hours, then Western blotting analysis was carried out to detect the expression of total or cleaved caspase-3/9. (F) MTT assay was conducted to test the cell growth after NK-92 cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 1, 2, 3, 4, 5, or 6 days. (G) NK-92 cells were administered 0, 20, 40, 80, or 160 μM of forskolin for 48 hours, then MTT assay was used to evaluate the IC₅₀ of forskolin. (H) NK-92 cells were incubated with 0, 20, 40, 80, or 160 μM of forskolin for 48 hours, then Western blotting analysis was carried out to detect the expression of total or cleaved caspase-3/9 (n=3, *P<0.05, ***P<0.001, forskolin group vs control group).

Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; NHL, non-Hodgkin’s lymphoma.