Figure 6.

SP600125 enhanced cell apoptosis induced by forskolin in NHL cells.

Notes: (A, B) The expression of the total or cleaved caspase-3/9 was decided by Western blotting after Toledo or NK-92 cells were treated with SP600125 (20 μM) or not (n=3, **P<0.01, ***P<0.001, SP600125 group vs control group). (C) Flow cytometry was used to determine cell apoptosis rates after Toledo or NK-92 cells were treated with SP600125 (20 μM) or not (n=3, *P<0.05, SP600125 group or forskolin group vs control group; ^#P<0.05, forskolin + SP600125 group vs forskolin group). (D, E) Toledo and NK-92 cells were treated with forskolin (40 μM), SP600125 (20 μM), forskolin (40 μM) + SP600125 (20 μM), or nothing, then cells were collected and the protein expression of cytoplasmic and nuclear β-catenin was examined by Western blotting (n=3, *P<0.05, SP600125 group or forskolin group vs control group; ^#P<0.05, forskolin + SP600125 group vs forskolin group). (F) Toledo and NK-92 cells were treated with forskolin (40 μM), SP600125 (20 μM), forskolin (40 μM) + SP600125 (20 μM) or nothing, then IP assay was conducted to measure the ubiquitination of Axin protein.

Abbreviation: GAPDH, glyceraldehyde 3-phosphate dehydrogenase.