Table S1.
Primer sequences of the genes used for RT-PCR.
| Gene | Sequence | Annealing temperature | References |
|---|---|---|---|
| Caspase-3 | R: CAAGCTTGTCGGCATACTGTTTCAG F: TTCAGAGGGGATCGTTGTAGAAGTC |
52°C | Hanif et al,1 2014 |
| Caspase-6 | R: AATTGCACTTGGGTCTTTGC F: ATCCTCACCGGGAAACTGTG |
54°C | Alharbi,2 2015 |
| Caspase-8 | R: GGATGAGGCAGACTTTCTGC F: GGCCTCCATCTATGACCTAG |
58°C | This study |
| Caspase-9 | R: GCACCACTGGGGGTAAGGTTTTCTAG F: ATGGACGAAGCGGATCGGCGGCTCC |
60°C | Krisnamurti et al,3 2016 |
| BAX | R: GGCCCCAGTTGAAGTTGC F: AAGAAGCTGAGCGAGTGTC |
54°C | Hanif et al,1 2014 |
| BAK | R: CCTGAGAGTCCAACTGCAAA F: GGTCCTGCTCAACTCTACCC |
60°C | Guan et al,4 2010 |
| BCL-2L1 | R: ATGGTCAGTGTCTGGTCATT F: TTGTGGAACTCTATGGGAAC |
57°C | Choi et al,5 2015 |
| p53 | R: CTCTCGGAACATCTCGAAGCG F: GCTCTGACTGTACCACCATCC |
57°C | Suo et al,6 2015 |
| HRK | R: CCCCCRAAAATTAAAAAAAAAACT F: GAGTTGAATTTAGGAAAAGGGGAA |
58°C | Zaker et al,7 2016 |
| Osteopontin | R: ACGGCTGTCCCAATCAGAAG F: AGCAGAATCTCCTAGCCCCA |
58°C | This study |
| VEGF | R: GCTTTCGTTTTTGCCCCTTTC F: CAACATCACCATGCAGATTATGC |
57°C | Wang et al,8 2015 |
| COX-2 | R: GCATCTGGCCGAGGCTTTTCTAC F: TGTGGGGCAGGAGGTCTTTGGTCT |
57°C | Kim et al,9 2011 |
| MMP-2 | R: GGCATCCAGGTTATCGGGGA F: GGCCCTGTCACTCCTGAGAT |
59°C | Benus et al,10 2005 |
| MMP-9 | R: GCACAGTAGTGGCCGTAGAA F: CTATGGTCCTCGCCCTGAAC |
54°C | This study |
| GAPDH | R: GGTCTACATGGCAACTGTGA F: ACGACCACTTTGTCAAGCTC |
59°C | Shi et al,11 2012 |
Abbreviation: MMP, matrix metalloproteinases.