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. 2019 Feb 19;11:1641–1651. doi: 10.2147/CMAR.S177508

Figure 5.

Figure 5

Upregulation of miR-140-5p inhibited the proliferation and promoted the apoptosis of G401 and SK-NEP-1 cells.

Notes: (A) RT-PCR analysis of the expression of miR-140-5p in normal kidney cell line HK-2 and WT cell lines, G401 and SK-NEP-1 (n=3, ***P<0.001). Next, miR-140-5p mimic, mimic-NC, miR-140-5p inhibitor, and inhibitor-NC were transfected to G401 and SK-NEP-1 cells, and then (B, C) CCK-8 assay was used to evaluate cell proliferation. (D) Flow cytometry with Annexin V/PI double staining was used to assess cell apoptosis (n=3, *P<0.05, miR-140-5p inhibitor group vs inhibitor-NC group; #P<0.05, miR-140-5p mimic group vs mimic-NC group).

Abbreviations: NC, negative control; RT-PCR, real-time PCR; WT, Wilms’ tumor; CCK-8, cell counting kit-8; PI, propidium iodide.