Figure 7.

miR-140-5p combined with TGFBR1 and negatively regulated its expression.

Notes: (A) The combining sequence between miR-140-5p and TGFBR1 gene. (B, C) G401 cells were co-transfected with WT or MT together with miR-140-5p mimic, mimic-NC, miR-140-5p inhibitor, or inhibitor-NC for 48 hours, and then dual-luciferase reporter assay system was used to assess the luciferase activity (n=3, *P<0.05, miR-140-5p inhibitor group vs inhibitor-NC group; ^#P<0.05, miR-140-5p mimic group vs mimic-NC group). (D). Western blotting was performed to analyze the protein expression of TGFBR1 in Wilms’ tumor tissues and the adjacent non-tumor tissues (n=3, *P<0.05, Wilms’ tumor group vs control group).

Abbreviations: NC, negative control; WT, Wilms’ tumor; 3′ UTR, 3′ untranslated region.