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. 2019 Feb 19;11:1653–1665. doi: 10.2147/CMAR.S188248

Figure 4.

Figure 4

Dysregulation of FGG affects the migration and invasion of HCC cells through EMT.

Notes: (A) Western blots showed the changes in E-cadherin, N-cadherin, Slug, and ZEB-1 expression after artificial modification of FGG expression. siFGG means knockdown of FGG with small interfering RNA duplexes. (B) Quantitative analysis of EMT-associated protein expression in SK-HEP-1 cells; (C) Western blot results showed that Slug and ZEB-1 were knocked down in SK-HEP-1 cells with FGG overexpression. (D) The migration and invasion of SK-HEP-1 cells with FGG overexpression were dramatically inhibited by the knockdown of Slug and ZEB-1. In this experiment, siSlug-3 and siZEB-1–3 were used to knockdown the expression of Slug and ZEB-1, respectively. Each experiment was independently performed at least three times. Data are shown as mean ± SD. *P<0.05, **P<0.01, ***P<0.001.

Abbreviations: EMT, epithelial to mesenchymal transition; FGG, fibrinogen gamma chain; siFGG, knockdown of FGG with small interfering RNA duplexes; NC, negative control; NS, non-significant; siSlug-1, siSlug-2, and siSlug-3, knockdown of Slug with small interfering RNA duplexes-1, duplexes-2 and duplexes-3; siZEB-1-1, siZEB-1-2, and siZEB-1-3, knockdown of ZEB-1 with small interfering RNA duplexes-1, duplexes-2, and duplexes-3.