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. 2018 Feb 27;18:230. doi: 10.1186/s12885-018-4151-x

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — A confocal image of double immunofluorescence labeling of tryptase and SSEA3. The image is a “maximum intensity” digital composite from 12 focal planes showing merged channels from dIF labeling of tryptase (green) and SSEA3 (red), together with DAPI nuclear staining (blue). Double-labeled tryptase+ SSEA3+ round/oval cells (thin arrows) and a SSEA3+ degranulated cell (thick arrow) are indicated. Scale bar 10 μm