Table 2.
Primary antibodies used for simultaneous visualization of two antigens by double immunofluorescence or single immunohistochemical labeling of individual antigens
| Epitope | Host species | Product code | Dilution IHC | Dilution dIF | Manufacturer |
|---|---|---|---|---|---|
| ALDH1 A1 | Mouse (IgG) | 611,194 | 1:1000 | 1:100 | BD |
| SSEA-3 | Rat (IgM) | MC-631 | 1:100 | 1:50 | DSHB |
| Tryptase | Rabbit (IgG) | Ab134931 | 1:2000 | 1:2000 | Abcam |
| Vinculin | Rabbit (IgG) | SAB4503069 | 1:300 | Sigma-Aldrich |
MC-631 (SSEA-3) was deposited to the DSHB by Solter, D. / Knowles, B.B
Abbreviations: dIF double immunofluorescence, IHC immunohistochemical labeling, BD Becton Dickinson Bioscience (Franklin Lakes, NJ, USA), DSHB Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA, USA); Abcam (Cambridge, UK); Sigma-Aldrich (St. Louis, MO, USA)