Table 3.
Statistically significant results for the model incorporating both intensity and duration of smoking
| CpG site | CHR | Gene or position | Intensity of smoking coefficient | Duration of smoking coefficient | Intensity of smoking coefficient p-value | Duration of smoking coefficient p-value | EWAS coefficient for CpG site |
|---|---|---|---|---|---|---|---|
| cg09935388 | 1 | GFI1 | −1.15E-02 | −1.85E-02 | 9.21E-06 | 5.83E-03 | −5.18E-01 |
| cg08709672 | 1 | AVPR1B | −5.78E-03 | 4.31E-04 | 1.01E-06 | 8.49E-01 | −1.98E-01 |
| cg03329539 | 2 | 2q37.1 | −6.49E-03 | −2.87E-03 | 3.11E-05 | 2.63E-01 | −3.24E-01 |
| cg05951221 | 2 | 2q37.1 | −1.08E-02 | −5.97E-03 | 3.75E-09 | 9.87E-02 | −6.68E-01 |
| cg21566642 | 2 | 2q37.1 | −1.23E-02 | −7.27E-03 | 5.54E-07 | 1.35E-01 | −9.99E-01 |
| cg01940273 | 2 | 2q37.1 | −8.37E-03 | −4.38E-03 | 2.28E-08 | 1.58E-01 | −6.16E-01 |
| cg00501876 | 3 | CSRNP1 | −5.33E-03 | −1.89E-03 | 5.24E-06 | 3.29E-01 | −1.57E-01 |
| cg19859270 | 3 | GPR15 | −7.45E-03 | −7.58E-03 | 4.19E-05 | 6.36E-03 | −3.02E-01 |
| cg02657160 | 3 | CPOX | −7.25E-03 | −7.50E-03 | 2.45E-05 | 4.66E-03 | −1.79E-01 |
| cg23576855 | 5 | AHRR | −1.59E-02 | −1.16E-02 | 9.81E-08 | 2.47E-01 | −1.00E + 00 |
| cg05575921 | 5 | AHRR | −4.32E-02 | −1.78E-02 | < 2e-16 | 8.51E-02 | −2.38E + 00 |
| cg26703534 | 5 | AHRR | −6.54E-03 | −1.34E-03 | 2.61E-07 | 5.70E-01 | −3.39E-01 |
| cg25648203 | 5 | AHRR | −1.04E-02 | −6.48E-03 | 1.75E-07 | 1.02E-01 | −4.13E-01 |
| cg21161138 | 5 | AHRR | −1.09E-02 | −7.39E-03 | 8.01E-09 | 3.69E-02 | −4.72E-01 |
| cg02451831 | 7 | KIAA0087 | −6.99E-03 | −2.85E-03 | 3.05E-05 | 3.34E-01 | −1.74E-01 |
| cg10750182 | 10 | C10orf105 | −3.78E-03 | −2.89E-03 | 4.20E-05 | 5.33E-02 | −1.27E-01 |
| cg02743070 | 10 | ZMIZ1 | −4.56E-03 | −4.68E-04 | 2.30E-05 | 7.80E-01 | −8.71E-02 |
| cg03450842 | 10 | ZMIZ1 | −4.54E-03 | −2.07E-03 | 5.93E-05 | 3.01E-01 | −1.52E-01 |
| cg21611682 | 11 | LRP5 | −4.91E-03 | −1.35E-03 | 6.35E-06 | 5.14E-01 | −1.89E-01 |
| cg11660018 | 11 | PRSS23 | −5.21E-03 | −4.93E-03 | 5.94E-05 | 7.83E-02 | −2.33E-01 |
| cg13525276 | 14 | TSHR | 8.15E-03 | 4.73E-03 | 4.76E-05 | 1.68E-01 | 1.88E-01 |
| cg18625627 | 14 | TSHR | 8.46E-03 | 4.39E-03 | 3.17E-05 | 2.08E-01 | 1.85E-01 |
| cg01513913 | 14 | 14q32.33 | −4.72E-03 | −1.55E-03 | 3.25E-05 | 5.20E-01 | −1.09E-01 |
| cg23594345 | 14 | 14q32.33 | −9.68E-03 | −1.49E-04 | 6.14E-05 | 9.73E-01 | −1.99E-01 |
| cg01208318 | 14 | 14q32.33 | −8.63E-03 | −9.63E-04 | 4.21E-05 | 8.27E-01 | −2.18E-01 |
| cg23161492 | 15 | ANPEP | −7.55E-03 | −9.10E-03 | 4.46E-05 | 3.95E-02 | −2.73E-01 |
| cg13500388 | 16 | CBFB | −6.27E-03 | −2.77E-03 | 6.18E-06 | 2.16E-01 | −1.16E-01 |
| cg10062919 | 17 | RARA | −3.41E-03 | −1.41E-03 | 1.84E-05 | 3.15E-01 | −8.87E-02 |
| cg00968616 | 17 | CUEDC1 | 4.60E-03 | −1.90E-04 | 2.56E-05 | 9.10E-01 | 7.15E-02 |
| cg03636183 | 19 | F2RL3 | −9.68E-03 | −7.95E-03 | 1.80E-06 | 1.18E-01 | −6.63E-01 |
| cg15159987 | 19 | CPAMD8 | −5.66E-03 | 2.20E-03 | 3.70E-06 | 2.97E-01 | −1.66E-01 |
| cg21473814 | 19 | CRTC1 | 7.35E-03 | 4.00E-03 | 3.83E-05 | 2.16E-01 | 1.93E-01 |
| cg11554391a | 5 | AHRR | −5.25E-03 | −1.58E-02 | 9.14E-03 | 2.13E-05 | −2.20E-01 |
CHR: chromosome of the CpG site; EWAS: epigenome-wide association analysis
astatistically significant for duration of smoking
Legend: Intensity of smoking is given in average number of cigarettes per day, and duration of smoking is length of smoking habit in years. The EWAS coefficient presented represents the methylation difference between smokers and never smokers at baseline, based on only the baseline information. It is presented to show that for all significant coefficients, the effect directions of increased intensity or increased duration are consistent with the effect of smoking vs not smoking