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. 2018 Oct 11;49:105. doi: 10.1186/s13567-018-0600-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Western blot analysis of pre- and post- (day 41) vaccination antisera. Pre- and post- (day 41) vaccination antisera (1:5000 dilution) from horse 1 (lanes 2 and 3), horse 2 (lanes 4 and 5), horse 3 (lane 6 and 7) and horse 4 (lanes 8 and 9) were used to detect AHS 5 VLP proteins in a standard Western blot analysis. No AHSV proteins were detected in any of the pre-vaccination sera (PB) (lanes 2, 4, 6 and 8) but all four were detected in serum sampled at days 41 post-vaccination (D41) (lanes 3, 5, 7 and 9). Protein bands of AHS viral proteins VP2, VP3, VP5 and VP7 are indicated with arrows; colour pre-stained protein molecular weight marker (New England Biolabs, Massachusetts, USA) is in lane 1.