Table 1.
VP7 indirect ELISA results and virus neutralising antibody titers (vnts) of the pre-vaccination sera
| Horse | iELISA—S/P % | vnt |
|---|---|---|
| Horse 1 | Negative | Negative |
| Horse 2 | Negative | Negative |
| Horse 3 | 83 | Negative |
| Horse 4 | 34 | Negative |
| Horse 5 | Negative | Negative |
| Horse 6 | 35 | Negative |
| Horse 7 | 39 | Negative |
| Horse 8 | 44 | Negative |
Pre-vaccination sera of all 8 horses used in the experiment were assessed for AHSV antibodies (Ab) by indirect ELISA. High positive control serum pooled from vaccinated horses inoculated with live attenuated vaccine (bottle 1) obtained from Onderstepoort biological products (OBP), was used as a positive control. Sample/positive (S/P) percentage values less than 5.0 are classified as negative, S/P values greater than or equal to 5.0, but less than 10.0 are considered suspect, while S/P values greater than 10.0 are classified as positive for AHSV Ab. The horse sera were assayed for neutralisation capability against AHSV 5, AHSV 8 and AHSV 4 and all horses were shown to be negative against all three serotypes tested. Sheep serum from animals vaccinated with live AHS virus was used as a positive control.