Table 3.
Observed quantification cycle (Cq) values for Schmallenberg virus (SBV) in field-collected Culicoides. Includes associated Culicoides species characterisation and blood-feeding method (– indicates no sequence data available; Obsoletus complex includes C. obsoletus (Meigen) and C. scoticus Downes & Kettle)
| Species | BINa | Blood-feeding method | SBV Cq value | Culicoides DNA barcode | ||
|---|---|---|---|---|---|---|
| Sample ID | GenBank ID | BOLD process ID | ||||
| C. obsoletus (Meigen) | BOLD:AAO7718 | Membrane | 32.95 | TPI:ENT:#0000313 | KT186817 | CUSBV066–15 |
| Membrane | 32.84 | TPI:ENT:#0000314 | KT186816 | CUSBV067–15 | ||
| Membrane | 33.64 | TPI:ENT:#0000315 | KT186815 | CUSBV068–15 | ||
| Membrane | 31.00 | TPI:ENT:#0000316 | KT186814 | CUSBV069–15 | ||
| Membrane | 37.88 | TPI:ENT:#0000319 | KT186813 | CUSBV072–15 | ||
| Membrane | 37.44 | TPI:ENT:#0000320 | KT186812 | CUSBV073–15 | ||
| Membrane | 36.11 | TPI:ENT:#0000321 | KT186860 | CUSBV074–15 | ||
| Membrane | 38.10 | TPI:ENT:#0000322 | KT186859 | CUSBV075–15 | ||
| Membrane | 32.61 | TPI:ENT:#0000323 | KT186858 | CUSBV076–15 | ||
| BOLD:AAM6198 | Membrane | 31.92 | TPI:ENT:#0000325 | KT186863 | CUSBV078–15 | |
| C. scoticus Downes & Kettle | BOLD:AAZ3985 | Pledglet | 37.88 | TPI:ENT:#0000317 | KT186880 | CUSBV070–15 |
| Pledglet | 33.83 | TPI:ENT:#0000318 | KT186881 | CUSBV071–15 | ||
| Obsoletus complexb | – | Membrane | 34.00 | – | – | – |
| – | Membrane | 27.12 | – | – | – | |
| – | Membrane | 37.67 | – | – | – | |
| C. pulicaris (L.)b | – | Membrane | 28.17 | – | – | – |
| – | Membrane | 35.95 | – | – | – | |
aBarcode Index Numbers (BINs) [49] assigned within the Barcode of Life Database (BOLD) [39] for specimens collected within this study: specimens TPI:ENT:#0000248–TPI:ENT:#0000262; TPI:ENT:#0000264–TPI:ENT:#0000283; TPI:ENT:#0000285–TPI:ENT:#0000286; TPI:ENT:#0000288–TPI:ENT:#0000312 [GenBank: KT186808–KT186811; KT186818–KT186857; KT186861–KT186862; KT186864–KT186879]
bSpecies identification based on morphology only