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. 2018 Feb 27;18:3. doi: 10.1186/s12899-018-0037-z

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — Hepatic STAT3 and SMAD1/5/8 protein expression in Alk3^fl/fl; Alb-Cre and Alk3^fl/fl mice. Representative western blots (a) and quantitative analyses (b) of phospho-STAT3 and total-STAT3 from Alk3^fl/fl and Alk3^fl/fl; Alb-Cre mice 14 days after heat-killed Brucella abortus (BA) or saline injection. Two isoforms of phospho-STAT3 α and β (79/86 kDa) exist. Loading control is indicated by total STAT3 protein levels. c SMAD1/5/8 activation 14d after BA administration in Alk3^fl/fl (Cre-) and hepatocyte-specific Alk3 deficient (Alk3^fl/fl; Alb-Cre) (Cre+) mice fed an iron deficient diet. Western blots from liver proteins analysed with phosphorylated SMAD1/5/8, total SMAD1, and α-tubulin antibodies. Arrows indicate specific pSMAD signal. d Densitometric analyses of the ratio of SMAD1/5/8 to SMAD1