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. 2018 Dec 7;6(4):1–22. doi: 10.1080/21688370.2018.1546537

Figure 1.

Figure 1.

GDNF-mediated human BNB morphological changes following serum withdrawal. Representative phase contrast digital photomicrographs of visually confluent pHEndECs in vitro following initial seeding and culture in regular growth medium (A) and following serum withdrawal in basal medium (B) and basal medium containing 1 ng/mL GDNF (C) demonstrate an admixture of proliferating cuboidal-shaped (white arrows) and spindle-shaped cells prior to serum withdrawal. Diffuse spindle-shaped endothelial cells are characteristic following serum withdrawal, with endothelial layers appearing more organized 48 hours afterwards in the presence of GDNF (C) compared to basal conditions (B). Intercellular gaps (white asterisk) and more cuboidal cells with larger cytoplasm and more diffuse intracytoplasmic F-actin cytoskeletal filaments are more commonly seen prior to serum withdrawal (D). Spindle-shaped cells with more F-actin localization at intercellular membranes (white arrows) are more prevalent following serum withdrawal (E, F) with fewer intercellular gaps (white asterisk) seen following GDNF treatment 48 hours afterwards. PC = Phase Contrast. Initial magnification 100X (A-C) and 400X (D-F).