Figure 3.

GDNF effect on transendothelial electrical resistance. A representative graph of continuous human BNB TEER measured by ECIS obtained from a single experiment shows the uniform gradual increase in TEER in regular growth medium. Serum withdrawal is associated with an initial increase in resistance that peaks in ~ 16–20 hours with progressive decline until the end of the experiment 48 hours afterwards under basal conditions. GDNF results in a higher TEER that peaks ~ 26–27 hours after serum withdrawal with less decline 48 hours afterwards. Specific inhibitors against RET-tyrosine kinase (RET-TK Inh), MEK1 (MEK1 Inh) and ERK1/2 (ERK 1/2 Inh) abrogate the GDNF-mediated TEER changes.