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. 2018 Dec 7;6(4):1–22. doi: 10.1080/21688370.2018.1546537

Figure 4.

Figure 4.

GDNF effect on BNB TEER recovery following serum withdrawal. Bar histograms demonstrate that GDNF significantly enhanced human BNB restrictive biophysical properties based on maximal calculated absolute TEER increase (A) and % maximal TEER (persistence) at 48 hours following serum withdrawal (B). Inhibitors against MEK1 (MEK1 INH) and ERK1/2 (ERK 1/2 INH) completely abrogated the GDNF-mediated resistance enhancement with no significant differences compared to basal conditions without added GDNF, while an inhibitor against RET-tyrosine kinase (RET-TK INH) resulted in a small, but statistically significant partial effect relative to basal conditions. *** indicates p < 0.001, **** indicates p < 0.0001 and n.s. indicates not significant. N = 3 independent experiments in triplicate.