Table 1.

Comparative quantitative proteomics of selected junctional complex-associated proteins. Proteomic quantification of selected proteins based on normalized average total ion current of cytosolic and membrane protein fractions of confluent pHEndECs 48 hours following serum withdrawal without (basal) and with GDNF treatment shows a total increase in CTNNA1 expression associated with a reduction in cytosolic expression and increase in membrane expression mediated by GDNF. There is a diffuse increase in TLN1 cytosolic and membrane expression following GDNF treatment compared to basal conditions. Total ZYX expression was reduced following GDNF treatment associated with reduction in membrane expression with minimal change in cytosolic expression compared to basal conditions. Protein extracts from 2 independent experiments were combined, quantified in triplicate and averaged using the Scaffold software as described in the Materials and Methods section.

Protein	Basal cytosolic	GDNF cytosolic	Fold Change	Basal membrane	GDNF membrane	Fold Change	Basal Total	GDNF Total	Fold change
CTNNA1	50798	39195	0.77	116810	145520	1.25	167608	184715	1.10
TLN1	86205	96750	1.12	119360	146880	1.23	205565	243630	1.18
ZYX	278630	269960	0.97	391860	233520	0.60	670490	503480	0.75