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. 2018 Oct 10;49:104. doi: 10.1186/s13567-018-0598-7

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Development of BALT regions in the intrapulmonary primary and secondary bronchi of CSF1R-transgenic birds. Whole mount analysis of the intrapulmonary bronchi of a CSF1R-mApple chicken. BALT structures are associated with each opening of the secondary bronchi and increase in size and number with age (A–L). Intrapulmonary bronchi of older CSF1R-eGFP animals show the presence of BALT (K–L) and the number of lymphoid follicles seemingly reduces in older animals (K–L). Primary bronchus (PB) is indicated by dashed lines, lymphoid follicles indicated by a dashed circle and openings to the secondary bronchi are marked by an asterisk. w = week/s of age.