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. 2018 Feb 26;17:31. doi: 10.1186/s12934-018-0859-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — RT-PCR analysis for heterologous expression of syl gene cluster. RNA was isolated from the growth phase of syl/M145 and syl/TK24 cultures. Lanes 2–4 show the successful amplification of syl gene cluster from recombinant cultures. Lane 5 shows the amplification of the 16S rRNA internal reference