Table 1.

Published alternative scaffold proteins and enzymes which have been engineered using yeast surface display

Engineered scaffold or enzyme	References
Fn3 domain of fibronectin	Lipovsek et al. [24] Koide et al. [52] Hackel et al. [51] Koide et al. [40] Hackel et al. [55] Mann et al. [57] Sha et al. [58] Heinzelman et al. [59] Park et al. [49]
Human kringle domains	Lee et al. [25] Lee et al. [66]
DARPins	Schütz et al. [70]
Cystine-knot miniproteins	Silverman et al. [74] Silverman et al. [75] Kimura et al. [76] Lahti et al. [77] Kimura et al. [78] Glotzbach et al. [79] Maaß et al. [80]
Sso7d	Gera et al. [84] Gera et al. [85] Traxlmayr et al. [86] Kauke et al. [87]
T7 phage gene 2 protein (Gp2)	Kruziki et al. [89] Chan et al. [90]
Pyrazinamidase	Strauch et al. [91]
vNAR domains	Zielonka et al. [26] Zielonka et al. [95] Könning et al. [27] Könning et al. [96]
VHH domains	Ryckärd et al. [110]
Variable lymphocyte receptor (VLR)	Tasumi et al. [14] Hong et al. [114]
Horseradish peroxidase	Lipovsek et al. [122] Antipov et al. [123]
Sortase A	Chen et al. [124]
Lipases	Han et al. [125] White and Zegelbone [126]
β-lactamase	Cohen-Khait and Schreiber [127] Cohen-Khait et al. [128]
Glucose oxidase	Ostafe et al. [129]
Nonribosomal Peptide Synthetase Adenylation Domain	Zhang et al. [130]
Tobacco Etch Virus protease	Yi et al. [131]