Table 1. Comparison of SV release.
| Tonic release | Evoked EPSC | RRP | Pvr | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| mEPSC (0 mM Ca2+) | mEPSC (1 mM Ca2+) | mIPSC (0 mM Ca2+) | mIPSC (1 mM Ca2+) | |||||||||
| Frequency (Hz) |
Amplitude (-pA) |
Frequency (Hz) |
Amplitude (-pA) |
mIPSC (Hz) |
Amplitude (pA) |
mIPSC (Hz) |
Amplitude (pA) |
Amplitude (-nA) |
Charge (-pC) |
Charge (-pC) |
||
| Wild-type | 1.87 ± 0.16 | 23.2 ± 1.6 | 43.5 ± 4.3 | 23.9 ± 1.1 | 11 ± 1.1 | 25.9 ± 1.1 | 42.5 ± 4.1 | 22.5 ± 2.1 | 2.27 ± 02. | 20.6 ± 2.1 | 221 ± 23.1 | 0.12 ± 0.01 |
| unc-13(s69) | 0.05 ± 0.05 | 20.2 ± 3 | 2 ± 1 | 21.1 ± 2.1 | 0.05 ± 0.05 | 22.1 ± 4.1 | 1.1 ± 0.5 | 21.5 ± 3.2 | 0.02 ± 0.01 | 0.05 ± 0.01 | 25.5 ± 7.1 | 0 |
| UNC-13L | 1.51 ± 0.45 | 19.3 ± 1.9 | 43.4 ± 3.7 | 23.9 ± 1.1 | 10.1 ± 0.9 | 22.3 ± 2.2 | 38.1 ± 3.5 | 22.4 ± 1.1 | 1.7 ± 0.14 | 15.4 ± 2.6 | 237 ± 25.4 | 0.06 ± 0.01 |
| UNC-14LΔC2A | 0.64 ± 0.07 | 20.5 ± 1.4 | 20.2 ± 3 | 23.3 ± 2.2 | 5.4 ± 0.5 v | 28.2 ± 1.9 | 34.4 ± 3.2 | 25.7 ± 1.8 | 1.08 ± 0.12 | 8.4 ± 1.6 | 248 ± 66.3 | 0.034 ± 0.006 |
| UNC-13L(K49E) | 1.9 ± 0.48 | 19.4 ± 1.1 | 43.3 ± 4.3 | 19.3 ± 2.4 | 9.8 ± 1.2 | 23.4 ± 1.5 | 40.6 ± 5.1 | 23.7 ± 3.2 | 2.1 ± 0.27 | 17.3 ± 2.3 | 243 ± 38.3 | 0.07 ± 0.01 |
| unc-10(K77/79E) | 0.57 ± 0.1 | 20.2 ± 1.8 | 45.4 ± 3.7 | 21.1 ± 1.2 | 3.5 ± 0.5 | 27.3 ± 1.8 | 39.7 ± 4.1 | 26.7 ± 1.1 | 1.18 ± 0.11 | 11.5 ± 1.5 | 245 ± 28.2 | 0.046 ± 0.006 |
| K49E;K77/79E | 0.94 ± 0.18 | 23.1 ± 1.5 | 40.8 ± 4.4 | 22.2 ± 1.6 | 9.5 ± 1 | 26.3 ± 1.4 | 43.7 ± 6.3 | 26.5 ± 1.8 | 1.81 ± 0.15 | 18.1 ± 2.2 | 260 ± 40 | 0.07 ± 0.008 |
All data were collected from three independent experiments to ensure the observed phenotype are replicable.