Fig. 1.

SLC4A11 expression in plasma membrane and H⁺ Flux. (A) Shows a representative western blot image of biotinylated SLC4A11 and (Na⁺/K⁺)-ATPase, selected from three independent experiments (n = 3). Procedure validation was performed by probing biotinylated and non-biotinylated fractions for GRP94, an ER resident protein, and cytosolic GAPDH. Percent in membrane fraction and (SD) are shown. (B) Plot of SLC4A11/(Na⁺/K⁺)-ATPase ratio for WT and each mutant showing the individual ratios from each experiment, the mean and SD, p>0.05. (C) The measurement of intracellular pH in PS120 cells. Average pHi traces from three or more independent experiments as indicated. Pulse with 10 mM NH₄Cl was maintained for 300 seconds. (D) Baseline pHi. (E) H⁺ efflux during initial 10 seconds of NH₄Cl pulse. (F) H⁺ influx in the presence of NH₄Cl (between Pulse and Stop). (G) Total acidification which is the difference between baseline pHi and lowest pHi after removing NH₄Cl. The values represent the average in n=3 tests and are shown as mean±SD. One-way ANOVA test in a comparison to WT, * indicates p < 0.05 and ** indicates p < 0.01.